Ministerio de Ciencia e Innovación

Colaboraciones intramurales

Las colaboraciones intramurales son líneas de trabajo entre, al menos, dos grupos CIBER, coordinadas por un grupo del CIBER-BBN que plantean un trabajo de investigación enfocado a una patología concreta y que dan respuesta a una necesidad clínica. Proporcionan una estructura reguladora para la actividad científica colaborativa de los grupos del CIBER-BBN en el contexto de las líneas prioritarias de investigación del centro según se definen en su programa científico.

Las colaboraciones actualmente en vigor se reflejan a continuación:

ACRÓNIMOTítulo del proyectoIPDescripción


Targeting triple negative breast cancer stem cells with nanomedicines

Ibane Abasolo

1. Potentially proprietary peptide sequences, selective to triple negative breast cancer stem cells.
2. CSC-targeted drug delivery systems with improved selectivity and efficacy in triple negative breast cancer in vitro and
in vivo models.


New bone targeted enzyme replacement therapies for pycnodysostosis

Ibane Abasolo

- Polymeric micelles based on pluronic-F127:COOH targeted against RANK
- Targeted exosomes with Cathepsin K expressing RANKL


Improved drug delivery systems for enzyme replacement therapies in Fabry disease. Effect of targeting, and
protein corona in the efficacy of nanosystems

Ibane Abasolo

1. Kwnoledge in the effect of targeting moieties on the effecacy internalization and biodistribution of GLA protein.
2. Optimization on the nanoliposomal formulation of GLA in terms of targeting moeities
3. Potential new formulation of GLA, including PEGylation and targeting moietites alternative to RGD


Increase of the stability, activity and crossing of biological barriers of recombinant proteins through their
vehiculization by EXtracellular vesicles and their PotentiaL biOmedical and industRial usE

Ibane Abasolo

1. EVs capable of deliverying lysosomal, cytoplasmatic and membrane proteins
2. Proof-of concept on the use of EVs for the delivery of membrane transporters (NPC1, CFTR)
3. Elucidation of the BBB-crossing mechanisms of Evs


Peptide-targeted polymeric micelles for an optimized cell internalization and anti-cancer treatment

Ibane Abasolo

proof-of-concept assays on the use of F7 as cell penetrating peptide in polymeric nanoparticles (PM). Such dossier will favor the licensing of the patent to a third party.


High resolution tracking of Extracellular Vesicles functionalized with METALlic nanoparticles

Jaquin Seras-Franzoso (Abasolo)

Techonological approach for the labeling of EVs with metallic particles in high resolution biodistribution analysis


Tumor extracellular vesicles as nanobeacons for personalized diagnosis of triple negative breast cancer (TNBC)

Jaquin Seras-Franzoso (Abasolo)

a diagnostic and prognostic system for triple negative breast cancer from blood samples. Particualry, the system will identify subsets of tumor extracellular vesicles (tEV) from blood samples using tailored single domain antibodies (sdAb) for their specific recognition and thermal lateral flow technology for their quantification


Treatment of aggressive Gliomas using Nanoparticulate systems for Blood-Brain-Barrier Crossing and Cancer Stem Cells Targeting

Fernanda da Silva Andrade (Abasolo)

The development of drug delivery systems based on nanotechnology able to cross blood-brain barrier (BBB) and specifically target cancer stem cells (CSC) subpopulation in combination with conventional therapy should allow more specific and effective therapies through the significant reduction of cancer progression and tumor relapse. Therefore, a positive clinical impact would be expected with this approach since an increase of patient quality-of-life and survival is predictable.
It is foreseen that the developed formulation and the know-how acquired during the project will allow the creation of a platform to produce a versatile nanocarrier able to efficiently cross the BBB giving origin to new therapies for GBM treatment, particularly in the prevention of CSC-driven cancer recurrence, allowing superior clinical outcomes.
Importantly, the proposed approach could be translated and applied to other brain malignancies considered clinical unmet needs due to low BBB crossing of the used drugs.
Moreover, the anti-CD147 and anti-CD133 peptipes created during the project could be useful to other applications, namely for diagnostic purposes.


Development of a Smart Hydrogel for Periodontal Regenerative Medicine Applications

Fernanda da Silva Andrade (Abasolo)

Currently, the treatments available for periodontal disease are very limited and often surgery is required. An appropriate treatment for periodontitis should combine anti-inflammatory, antiseptic, and tissue regeneration therapy, but the currently marketed formulations only respond to one of the three functions. In addition, they are excessively fluid and the time of permanence is very limited due to the constant renewal of saliva.
The proposed project aims to combine a potent anti-inflammatory (dexamethasone), a next-generation antiseptic that does not cause dental staining effects (cetylpyridinium chloride, CPC) and an agent with regenerative properties, such as hyaluronic acid. In addition, thanks to the use of thermo- and photosensitive polymers, a controlled deposition and its solidification can be achieved using a LED lamp commonly used in dentistry.
Therefore, the resulting formulation seems easily patentable and, in addition, it can be a vehicle to administer other drugs of interest for other dental pathologies.


Polymeric nanoparticles for the treatment of bacterial diseases

María Rosa Aguilar

Bacteriophage endolysins encapsulated in amphiphilic chitosan nanoparticles that incorparate silver nanoclusters


Protein-functionalized nanoparticles as serotype-independent pneumococcal vaccines

María Rosa Aguilar

Platform of protein-functionalized nanoparticles as serotype-independent pneumococcal vaccines


New therapeutic clinical solutions for the treatment of damaged radiated skin

María Rosa Aguilar

1.1. Library of cannabinoid-laden NPs with therapeutic properties
1.2. Collagen solutions loaded with peptides, NPs, dendrimers or adenoviruses for use in radiated skin treatment
1.3. Dressings, membranes and sponges loaded with peptides, NPs, dendrimers or adenoviruses for their use in the treatment of radiated skin
2.1. Obtaining cannabis extracts for the treatment of skin damage
3.1. Manufacture of dendrimers and dendritic gels with antioxidant and antibacterial activity
3.2. Design of a skin model for the study of radiation effects
4.1. Design of in vitro models for the study of the properties of molecules and bioactive systems
5.1. Design of an in vivo model for the study of the effects of radiation and its treatment


Multivalent chemical tools to study GPCR oligomerization

Miriam Royo

Bivalent and multivalent ligands that interact specifically to dopamine (D2R, D1R) and adenosine (A2AR, A2BR, A1R) oligomer receptors with high affinity and tissular specificity


Gamma-peptides based on cis-4-amino L-proline as pharmacological therapy in Alzheimer's disease

Miriam Royo

Establishment of P33 g-peptide as specific BACE1 activity modulator to be administered alone or in combination with other drugs to be used in primary prevention or early secondary prevention stages.


Multifunctional drug delivery systems for the treatment of advanced colorectal cancer

Miriam Royo

1. Targeted and non-targeted monotherapy drug delivery systems of traditional chemotherapeutic or CSC-selective compounds for mCRC treatment.
2. Targeted and non targeted combination nanotherapy of traditional chemotherapeutic and CSC-selective compounds for mCRC treatment.


Protein delivery system based on non-covalent PEGylation with telodendrimers

Miriam Royo

A library of NTA/DPTA telodendrimers modified with different binding moieties (charged or hydrophobic) and complexed with BSA, GFP, a-galactosidase (GLA) proteins


Dendrimer decorated inorganic nanoparticles: development of dendronized nanosystems against infection

Blanca González (Arcos)

i) desing of antibiotic free nanosystems with antimicrobial
activity and ii) desing of nanosystems with targeting capacity to the bacteria or to the biofilm and to virus envelope


Drug delivery nanosystem for colorectal cancer

María Vallet (Arcos)

-Mesoporous silica nanocarriers able to target cancer cells
-Colorectal cancer organoids for drug activity assays


Design of nanoantibiotics against bone infection

Isabel Izquierdo Barba (Arcos)

viable, scalable and cost-effective solution to the current problem caused by infections associated with osteoarticular prostheses, specifically polymicrobial infections, in which different species of microorganisms can coexist, some of them potentially multi-resistan


NANOsystems based on Mesoporous nanoparticles and EXOSomal membranes for the treatment of amyloidosis derived from Type 2 Diabetes Mellitus

Daniel Arcos Navarrete

New technology for targeting the organs and tissues affected by human amylin deposits such as the heart, central nervous system, kidney, liver and muscle tissue.


Engineering on-demand protein coronas for improved NANOparticle drug Delivery to tumors

Montserrat Colilla Nieto (Arcos)

New technology that will enable the spontaneous and selective recruitment of a protein corona on the surface of antitumoral nanoparticles (NPs) upon systemic administration.


Multiparametric MR data processing and software strategies for cancer response biomarker discovery

Carles Arús

Bespoke software for the quick and user-friendly post-processing of conventional, diffusion, perfusion MRI and MRSI.


MR-based in vivo Biomarkers of immune system activity against Glioblastoma

Carles Arús, Ana Paula Candiota

MR-Based non-invasive derived biomarker of successful therapy response related to efficient immune system actuation


Point Of Care Biosensor Devices To Detect Biomarkers As Evaluation End-Points For Therapeutic Clinical Trials In Ocular Surface Inflammation

Amalia Enríquez de Salamanca (Calonge)

Electrochemical and optical immunosensor or microRNA-based PoC


Mesenchymal stem cells as a next generation drug delivery system for ocular surface diseases

Teresa Nieto (Calonge)

Drug delivery system using mesenchymal stem cells (MSCs) as carriers for delivering drugs to the ocular surface of patients with limbal stem cell deficiency


Development of an in vitro three-dimensional microenvironmental tumor model to identify therapeutic mechanisms in colorectal cancer

Elisabeth Engel

3D model that recreates mechanically and biochemically the tumor microenvironment


Bioengineered constructs based on Elastin-like recombinamers (ELRs) for cardiac regeneration

Elisabeth Engel

- Electrospun scaffolds which release lactate embedded in bioactive ELR hydrogels
- 3D bioprinted patches of ELRs with optimized properties through the blending of viscosity enhancers


Functionalization of DNA origami with the T22-GFP-H6 protein for the enhancement of antimetastatic properties.

Ramón Eritja

Development of molecular defined nanodrugs with specific targeting to metastasic cancer stem cells


Advanced non-viral vectors for the development of new therapeutic approaches for retinal degenerations

Eduardo Fernández

Design, development and validation of new therapeutic approaches for several retinal dystrophies and inherited retinal diseases based on smart non-viral vectors


Combining Niosome and Optogenetics Technologies to Photosensitize Neural tissue

Eduardo Fernández, Lawrence Humphreys

Nanoparticle formulations capable of delivering optogenetic plasmids in vitro and in vivo for therapeutic use


Dendritic carbosilane systems as anti-neurodegenerative agents against Parkinson’s disease

Rafael Gómez Ramírez

Designing and developing novel therapeutic strategies for fighting Parkinson disease, based on new nanopharmaceuticals. New drug with potential technology transfer, susceptible for clinical translation.


In vitro electromechanical stimulation of mesenchymal stem cells for osteogenic, chondrogenic or myogenic differentiation

José Luis Gómez Ribelles (Gallego)

Smart cell culture materials (flat sheets and 3D scaffolds of piezoelectric polymer containing hydrogels with growth factors) that can be electromechanically stimulated 


3D culture medium for multiple myeloma cells

José Luis Gómez Ribelles (Gallego)

3D culture matrix consisting of copolymer (EA, EMA, AAc) microspheres coated with fibronectin, collagen type I, hyaluronic acid or chondroitin sulphate


Bactericidal effect of the modulation of the surface density of electric charge on hybrid surfaces of titanium and copolymers based on poly(vinylidene fluoride)

José Luis Gómez Ribelles (Gallego)

-HA-Ag multilayer deposited on a TiN-Ti titanium alloys
- Hybrid surfaces of titanium and a piezolectric polymer (p(VDF-TrFE)) with modifiable electric charge surface density


Characterization and resistance to microbial colonization of biodegradable magnesium based material

Mª Luisa González

PLA/Mg alloy composites


Novel PLA films including quercetin

Mª Luisa González

Development of an improved PLA to manufacture different degradable prosthetic materials with enhanced biocompatibility and response to infection over a lifetime of the device


Upstream-targeted molecular prosthetics for vision restoration

Pau Gorostiza

Small photoswitchable molecules to restore sight


Photopharmacological control of heart rate

Pau Gorostiza

Small molecules targeting cardiac subtype muscarinic acetylcholine receptors with dual photochromic and pharmacological activity that transduce externally applied illumination into heart rate changes


In vitro and in vivo characterization of light-regulated antitumoral compounds

Pau Gorostiza

Light regulated drugs against tumors


The nanoconductance of redox proteins of the respiratory chain and its physiopathological implication in leukemia

Pau Gorostiza

Correlation model between the alterations in the electron transfer between redox partner proteins of the mitochondrial respiratory chain and in-vitro assays targeting leukemia stem cells 


Membrane biophysics in lysosomal storage disorders for improved therapies

Pau Gorostiza

establishment of a series of mehcnaomarkers, and the rationale for the design of effective treatments, for lysosomal storage
disorders, like Fabry disease, among others. We forsee the establishing of a platform for the evaluation of the bioactivity at
the membrane level of enzymes and delivery systems for ERT in such diseases


Development of new nanoparticles and protocols for enhanced hyperthermia

José Javier Serrano (Hernando)

- Tumor associated macrophages type 1 (M1) containing gold optical nanoparticles  and micro-/nano-particles made of iron oxides and silica
-New instruments and animal models for application of Hyperthermia as cancer treatment


Development of technical tools for helping the movility of blind people

José Javier Serrano (Hernando)

An augmented reality application that uses multisensory interfaces to help people with visual disabilities or frailty


Bioengineering and artificial intelligence to improve diagnosis and prognosis of cardiovascular diseases in clinical practice

María Elena Hernando

Creation of digital tools to improve diagnosis and prognosis of patients with cardiovascular diseases based on
prediction models and automatic processing of multiparametric data sources, including medical images, electronic health records and biomedical signals.


Analysis of overnight heart rate variability to help in sleep apnea-hypopnea syndrome characterization and diagnosis

Roberto Hornero

Algorithms for characterizing and/or screening sleep apnea (biomarkers).


Explanatory and predictive models of failure in Transcatheter Aortic Valve Implantation (TAVI) using Big Data analysis techniques

Roberto Hornero

Tool to predict failure in TAVI
intervention using machine learning techniques and data mining approaches, such us those based on weighted
correlation networks


Development of non-invasive electrodes for long-term electroencephalography (EEG) recording with application in brain-computer interfaces

Roberto Hornero

A validated EEG recording system based on wearable temporary tattoo
electrodes (TTE) electrodes for sleep disorder monitoring and brain-computer interface applications.


Genetic analysis of sepsis and intraoperative neuromonitoring for the assessment of postoperative behavioral changes in children

Roberto Hornero

A tool for the automatic identification of genes relevant to sepsis, personalized for each user. This could provide a tailored treatment. A new operating protocol would be obtained in children that helps to reduce the disorders associated with it.


Introduction, development and support of electrophysiological signal acquisition for the assessment of rare diseases

Joan F. Alonso López (Jané)

Protocols for EEC and EMG non-invasive acquisition and processing that can infer disease related cerebral and muscular activity alterations


Developement of computational tools for training healthcare professionals in the use of mechanical ventilation and for continuous monitorization of critically ill patients

Miguel Ángel Mañanas (Jané)

A computational tool, MV-trainer, which can simulate the patient-ventilator interaction


Multimodal non-invasive monitoring and ventilation for COPD patients

Raimon Jané

Novel multimodal physiological biomarkers, based on advanced signal processing, to monitor COPD in a quantifiable, reliable and objective way


Multimodal mHealth tools for improving monitoring of Obstructive Sleep Apnea patients

Raimon Jané

Novel mHealth tools (sensors and software) for better monitoring and management of OSA patients


Neuromuscular signal analysis to decipher the reorganization of the spinal cord networks controlling the recovery of skilled hand function in rodents

Raimon Jané

Mathematical algorithms for neural and muscular signal analysis


Assessment of periodic breathing to improve diagnostic and treatment of chronic heart failure patients

Beatriz F.Giraldo (Jané)

Computational models and new clinical markers of cardio-respiratory decompensation of heart failure patients


Electrophysiological signal processing for the assessment of disabling neurological and psychiatric diseases

Joan F. Alonso López (Jané)

automatic software process electrophysiological data and it integration with clinical, cognitive and behavioural data useful for identify potential biomarkers in neurological and psychiatric diseases.


Early identification of severity in patients with COVID19 using acoustic cough analysis with smartphones

Raimon Jané

New tool using the microphones of smartphones to acquire and detect specific COVID19 cough patterns in a Health App Ecosystem


ECG derived cardiovascular risk for SCD and PFD in CHF patients

Pablo Laguna (Alba Martín Yebra)

ECG-based biomarkers able to discriminate death risk from SCD and PFD in chronic heart failure patients, aslo suitable for the atrial fibrillation cohort. A system/platform/tool to assess physical activity and physiological biomarkers (heart rate, respiratory rate, heart rate
variability, cardiorespiratory function) from the pulse photoplethysmographic signal (PPG) provided by a wrist-worn
wearable device, worn by the patient during 7 days.


Investigation on signal and image processing tools to improve the management and treatment of ventricular arrhythmias in the EP-lab

Juan Pablo Martínez (Laguna)

A system/platform/tool to assess and map cardiac substrate features related to arrhythmogenecity obtained from spatiotemporal


Characterization of the influence of physical activity on age-related cardiac function

Esther Pueyo (Laguna)

Software for ECG and accelerometry analysis and for characterization of circulating miRNAs reflecting changes in cardiac function related to physical activity and age.


Non invasive assessment of Autonomic Nervous System in intensive care units

Jesús Lázaro (Laguna)

Algorithms for ANS assessement for ICU patients that allow a better prognosis


Non invasive assessment of Autonomic Nervous System in depression

Raquel Bailón (Laguna)

A system/tool to assess depression evolution from autonomic nervous system markers derived from physiological signals


Follow-up of cardiovascular risk related to OSA through ANS evaluation

Eduardo Gil (Laguna)

Algorithms for characterizing and/or screening sleep apnea.


Multiscale characterization of the ventricular remodeling in diabetic cardiomyopathy for arrhythmic risk assessment and potential therapeutic effects of nutraceutical interventions with products of the Mediterranean diet

Pablo Laguna, Aida Oliván

In terms of technological development, the project will provide an optimized platform for biomimetic culture of cardiac tissue slices, with implementation of a sensor to monitor electromechanical stretch and culture environment. This platform has a great potential for translational scientific research by the generation of representative and useful cardiac disease models and also as a platform for drug testing and cardiotoxicity assessment.
The potential use of foods and compounds from the Mediterranean diet as a therapeutic antioxidant treatment in diabetic cardiomyopathy could contribute in the medium and long term to improve the quality of life and decrease the premature mortality of many diabetic patients. This will help to reinforce our healthcare system and reduce the economic burden on it. Since this is a highly prevalent disease, affecting millions of people worldwide, the socio-economic impact on our society is expected to be very high.
Additionally, it has the potential to improve valorization of local and national agro-food industry.


Physical activity and physiological biomarkers from wearable devices for personalized exercise medicine

Raquel Bailón (Laguna)

A system/platform/tool to assess physical activity and physiological biomarkers (heart rate, respiratory rate, heart rate variability, cardiorespiratory function) from the pulse photoplethysmographic signal (PPG) provided by a wrist-worn wearable device, worn by the patient during 7 days.


Polygenic scores associated with extreme values of HDLc

Julia Ramírez (Laguna)

More research is needed in order to know the main factors implicated in plasma HDLc levels. We would like to deeply describe the genetic factors involved in HDLc levels in subjects with extremely low and high HDLc concentrations. In this sense, polygenic risk scores are calculated by computing the sum of risk alleles corresponding to a phenotype of interest in each individual, weighted by the effect size estimate of the most powerful genome‐wide association studies (GWAS) on the phenotype. Therefore, we would like to generate a polygenic risk score that would explain the genetics related to extreme values of HDLc.


Rapid diagnosis of sepsis using ultrasensitive multiplexed photonic biosensors

Laura Lechuga

Α multiplexed photonic point-of-care biosensor for the early detection of specific biomarkers (CRP, IL-6, PCT, microRNAs and bacteria) relevant to sepsis in serum


Point-of-care optical device for antibiotic therapy tailoring and monitoring in nosocomial infections

Laura Lechuga

Photonic point-of-care biosensor devices for direct, fast and bedside antibiotic monitoring


Analysis of cancer metabolism and drug diffusion in 3D cell models

Luis Liz Marzán

SERS sensing platforms implemented in combination with cancer-on-a chip devices and/or 3D printed cancer models for in-situ non-disruptive spectra acquisition


Glycoengineered Exosomes as Vehicles for Gene and Drug Delivery

Niels Reichardt (Liz-Marzán)

Exosomes as drug delivery vehicles


Absolute quantification of glycans to distinguish between active and latent Tuberculosis

Niels Reichardt (Liz-Marzán)

Mass spectroscopy-based glycan analysis assay


Glycan biomarkers in thyroidal cancer

Niels Reichardt (Liz-Marzán)

New glycan serum biomarkers for thyroidal cancer stratification


3D bioprinting of an in vitro air-blood barrier for validating lung targeted drug nanocarriers

Dorleta Jimenez de Aberasturi  (Liz-Marzán)

- A two layer barrier system mimicking the alveolar-tissue made by 3D bioprinting of alveolar epithelial cells and lung endothelial cells
- Hybrid hydrogel lipid and polymeric capsules with different surface coatings and loaded with a model messenger RNA encoding a fluorescent probe


DEEP learning networks for Stratification of Cells by surfacE-Enhanced ramaN Scattering

Luis Liz-Marzán

Microfluidic platform device forHigh-throughput SERS screening assays.
Machine learning algorithms for estimating SERS-derived metabolites and analyzing spectroscopic data


Interactive platform for early detection of neurodegenerative diseases

José Miguel López Higuera

An interactive cognitive test for early detection of neurodegenerative diseases to be implemented into multimedia platforms


Hyperspectral and OCT imaging of muscle for assessing muscle degeneration and dystrophia in Duchenne disease

Olga Conde (López)

OCT, PS-OCT, HSI, SFDI techniques for neuromuscular degradation assessment


Fast Identification of caRbapEnemaSe-producing enTerObacteriaceae by RamaN spEctroscopy

Adolfo Cobo García (López)

A custom, optimized device based on Raman spectroscopy with improved sensitivity, coupled with machine learning algorithms, to perform fast identification of CPEs from a culture.


ORgan-on-ChIp For photonic characterization of living cElls

Adolfo Cobo García (López)

a platform based on photonic Lab-on-Chip technologies suitable for the growth of living cells in a controlled environment, i.e. a so-called organ-on-chip device with photonic characterization capabilities. The organon-chip will use transparent and biocompatible materials, such as glass, with particular microfluidic designs to favor cell growth dynamics and analysis. In addition, optical elements will be included to potentially detect, identify and characterize the living cells in a single chip using different photonic techniques, e.g. absorption, fluorescence and Raman spectroscopy


Targeted Nanoparticle Combination for the Selective Killing of Metastasis Stem Cells and Associated Fibroblasts in Colorectal Cancer

Ramón Mangues

Protein nanoparticles targeting FAPα+ Metastasis-Associated Fibroblasts


Improved colorectal cancer therapy using CXCR4-targeted functional protein nanoparticles with enhanced cytosolic release

Ugutz Unzueta (Mangues)

Development of tumor-targeted nanoparticles with enhanced cytosolic release that allows to avoid lysosomal proteolytic degradation


Site-directed conjugation of multivalent protein nanomaterials for precision medicine of cancer

Ugutz Unzueta (Mangues)

Development of a new generation of nanoconjugates platform based in site-directed conjugation technologies and with specific targeting for cancer stem cells.


CXCR4-targeted multivalent amanitin-nanoconjugates for precision medicines in colorectal cancer

Ugutz Unzueta (Mangues)

Development of a new CXCR4+ targeted amanitin nanoconjugate for selective blockade of RNA polymerase II in cancer stem cells.


Impact On Colorectal Tumor Metabolism When PDGFRβ Fibroblasts Are Depleted By Targeted Nanoparticles

Lorena Alba Castellon (Mangues)

Protein-based nanoparticle that eliminates a subpopulation of fibroblasts located in the stroma of the tumor. To determine whether this depletion changes the biology of the tumor, specifically its metabolism. These data will help us to understand the mechanism through which fibroblast-targeted nanoparticles exert their antitumor activity.


Immunochemical Diagnostic Strategies based on the Quorum Sensing System

María Pilar Marco, Núria Pascual

QS molecules as biomarkers of infection and the possibility to establish QS molecular fingerprints which may permit to distinguish infection from just colonization, assess the disease
severity, provide information about the prognostic or predict pulmonary exacerbations. Laboratory
methods (ELISA and microarray) and PoC easy-to-use immunosensors


Integrated strategies for the COVID19 diagnosis and disease progression

María Pilar Marco

Innovative technologies for the diagnostic and follow-up of viral infections will be delivered.
The knowledge generated and the technologies developed while trying to develop diagnostic technologies to detect
genetic material and structural proteins of the SARS-CoV2 have laid the foundations for addressing the detection of other viruses and bacteria.


Development of monoclonal antibodies against C99 fragment of APP protein and study of its therapeutic properties for the Alzheimer’s disease treatment

María Pilar Marco, Núria Pascual

Monoclonal antibodies against APP


Circulating tumor cells as a prognostic marker of chemo- and immunotherapy resistance

Ignacio Ochoa (Martínez Barca)

-Microfluidic chips capable to isolate circulating tumor cells and clusters
-Magnetic nanoparticles functionalised with specific antibodies against circulating tumor cells


Mechano-biological study of the extracellular matrix rigidity impact in chronic heart failure

Ignacio Ochoa (Martínez Barca)

Model for myocardium fibrosis


Mimicking hypoxia in glioblastoma and malignant melanoma with microfluidic chips: targeting PARP to blunt the hypoxic response

Ignacio Ochoa (Martínez Barca)

Microfluidic chip for 3D tumor cells culture


Evaluation and validation of cryopreservation as a storage method for encapsulated cells used in cardiac regenerative therapy

Ignacio Ochoa (Martínez Barca)

Cardiosphere derived cells encapsulated within alginate-polylisine-alginate microspheres


Human bioengineering for detection and analysis of cardiotoxicity in anti-neoplasic therapy

Estefanía Peña (Martínez Barca)

Human cardiotoxicity screening system to be employed in improving onco-cardiologic safety. This engineered tissue with optimal reproduction of the mechanical properties of native myocardium would be able to provide maturation signals to the hiPSC-derived cardiac cells (cardiomyocytes, cardiac fibroblasts, and endothelial cells) that will populate it.This advanced system will be utilized for testing oncotherapeutics to predetermine patient predisposition to cardiovascular toxicity


Computational biomechanics and bioengineering 3D printing to develop a personalised regenerative biological ventricular assist device to provide lasting functional support to damaged hearts

Manuel Doblaré (Martínez Barca)

1. To develop a data-to-design workflow for the fabrication of the structural component of BioVADs. This will require the complementary feedback between Cardiac Imaging, CompM and 3D printing.
2. To generate a personalized, mature and contractile BioVAD. Cellular reprogramming, hiPSC and composite materials will be the main players here.
3. To demonstrate safety and efficacy of their application in a clinically relevant model of IHD in pig. This will entail applying the latest cardiac imaging and surgery expertise.


Advanced multifunction 3D biofabrication for the generation of computationally modelled human-scale therapeutic cardiac tissues

Manuel Doblaré (Martínez Barca)

CARDIOPRINT is born with the ambition of shaping a quantum leap in the fields of Additive Manufacturing and Biofabrication of therapeutic human cardiac tissues, at both the technological and applicative levels. The overall concept of this enterprising project is to develop a new multifunctional additive manufacturing technology able to provide the sufficient accuracy for the manufacturing of human tissues at an organ scale for the first time. This will encompass fabricating new 3D printing devices able to attain superior cell-deposition precision and new cardio-mimetic polymers and hydrogels, but additionally, setting up the workflow to complete the cardiac image-to-transplant of organ sized mature bioartificial hearts (BioHearts). This novel combination of workflow and technological development has the potential to be extrapolated to other human tissues and pathologies. It will constitute a new therapy that will significantly improve the patients´ quality of life and reduce their dependence on medication and medical surveillance. This will in turn benefit our national care system by decreasing costs and human burden, and boost industrial growth in this area, driving Spain´s position towards leadership


Responsive actuators with Soft Robotic functions for microfluidics

Carlos Sánchez (Martínez de la Fuente)

Active valve technology at TRL4


Hydrogels with tunable mechanical properties for cancer treatment studies

Carlos Sánchez (Martínez de la Fuente)

Hydrogel platform with tunable mechanical properties


Doubly functionalised nanoparticles for the non-invasive characterisation of early, asymptomatic atherosclerosis

Lucía Gutiérrez (Martínez de la Fuente)

Possitive contrast agents based on magnetic nanoparticles designed for atherosclerosis detection


Nanoscale magnetic heating studies using cell membrane models

Raluca Fratila (Martínez de la Fuente)

A new methodology for studying the effect of localized magnetic heating on cell membrane models


Bioorthogonal chemistry strategies for the selective immobilization of magnetic nanoparticles on living cell membranes

Raluca Fratila (Martínez de la Fuente)

A new methodology for cell surface modification with nanomaterials


Development of new peptide-gated systems for controlled release applications

Ramón Martínez Máñez

Gated materials able to target specific cells


Targeting age-related vascular senescent cells with nanoparticles to improve cognitive function

Ramón Martínez Máñez

New clinical senolytic strategies based on hybrid nanodevices for targeting senescence-associated diseases.


New therapeutic nanosystems for Retinitis Pigmentosa based on fuctional mesoporous silica nanoparticles

Ramón Martínez Máñez

The purpose of the project is to obtain a nanosystem based on mesoporous silica nanoparticles for the administration of IL1β and TNFα small interfering RNA (siRNA), to control neuroinflammation during retinitis pigmentosa progression.


Molecular and metabolic biomarkers in bladder cancer for new sensing and treatment strategies

Ramón Martínez Máñez

Biomarkers for Bladder Cancer diagnosis and treatment monitoring by high resolution NMR spectroscopy of tissues (HRMAS) from animal models with different treatments.


Search for biomarkers of Alzheimer´s disease in serum

Ramón Martínez Máñez

Biomarkers for Alzheimer’s disease and Mild Cognitive impairment diagnosis and progression monitoring by high resolution NMR spectroscopy of serum from patients.


Development of gated bioglass and 3D scaffolds

Ramón Martínez Máñez

Scaffolds for selective and controlled cargo delivery based on 3D supports and gated mesoporous materials


Design of nanodispositives based on mesoporous silica nanoparticles for treatment of breast cancer

Ramón Martínez Máñez

new gated systems able to both detect and treat breast cancer. The prepared systems will increase the efficiency of the chemotherapy and will contribute to avoiding future relapses and metastasis


New strategies for cancer diagnosis and treatment based on mesoporous materials and glycans

Ramón Martínez Máñez

Gated nanoparticles with Lex as linker to cap with AAL lectin also targeting molecule


Development of oligonucleotide-gated nanodevices

Ramón Martínez Máñez

Oligonucleotide-capped materials that could be used as a diagnostic tool of selected biomarkers


Exploring the possibilities of mesoporous silica materials in cancer detection and treatment based on cancer secretome-related molecules

Ramón Martínez Máñez

Studies of long term nanoparticles toxicity and behaviour and new nanodevices based on cancer secretome-related molecules for cancer detection and treatment


Photo-activation of prodrugs using plasmonic nanoparticles

Ramón Martínez Máñez

Intracellular therapeutics based on properly designed non-toxic prodrugs, containing a photo-labile linker for cancer therapy. The prodrugs will be transformed in their active form by NIR irradiation thanks to gold nanostars


Gated nanomaterials for fighting chronic diseases

Ramón Martínez Máñez

development of nanodevices for the treatment of chronic diseases. On the one hand, the aim is to develop nanodevices for the healing of chronic wounds, using two stimuli-sensitive nanoparticles for the sequential administration of drugs through the inflammation and proliferation phases. On the other hand, other nanosystems will be developed for the elimination of senescent cells in ulcerative colitis, based on molecular probes and/or mesoporous materials loaded with senolytic drugs


Development of systems based on gated materials for assessing the risk to suffer cardiotoxicity associated to anthracycline treatment

Ramón Martínez Máñez

A new diagnostic tool for the detection of cardiotoxicity risk using via the use of gated materials to sense miRNA abundance in serum


Biomedical nanomotors for the treatment of fungal infections

Ramón Martínez Máñez

Novel biomedical nanodevices for the treatment of fungal infections


Development of new senolytic strategies for the detection and treatment of metabolic associated fatty liver disease

Ramón Martínez Máñez

New clinical senolytic strategies for the detection of senescence and treatment to induce regeneration after liver injury.


New technologies in organoid development: Platform for modeling infectious diseases. Nanotechnology-based  therapies

María Ángeles Muñoz Fernández

Hydrogel technology for human pluripotent stem cells (hPSCs) derived-organoid culture that will improve organoid culture efficiency and consistency. Human organoids platform for the study of human infectious diseases and nanoparticle research


Functionalization of polymer coatings using antimicrobial agents for prevention of mesh infection following hernia surgery

María Gemma Pascual

Functionalizing prosthetic meshes for hernia repair with innovative antimicrobial and bactericide coatings, to avoid postoperative infections in the implant and adjacent tissues


Abdominal prostheses and fixation mechanisms to improve adaptation to host tissue properties in hernia repair

María Gemma Pascual

New abdominal prostheses and fixation concepts to be used in the surgical repair of hernia pathologies. The goal is to produce pototypes with potential technology transfer, susceptible for clinical translation.


Preclinical application of carbosilane dendrimer systems for the prevention and treatment of bacterial infections in skin wounds

María Gemma Pascual

A novel, smart ointment formulation exerting antibacterial activity (cream functionalized with carbosilane dendrimer molecules) for topical application as a prophylactic strategy to avoid bacterial infections in cutaneous wounds


Developing smart 3D-printed antibacterial mesh coatings for hernia repair

María Gemma Pascual

Developing, via 3D-printing technology, an antibacterial biopolymer compound provided with drugs, for the prophylactic coating of hernia repair mesh materials. The goal is to create an innovative and highly efficient antibacterial mesh device with potential technology transfer, susceptible to clinical translation.


Development of a non-viral genome-editing platform based on CRISPR/Cas9 and autoassembled lipid nanoparticles to correct genetic mutations in rare diseases

Gustavo Puras Ochoa (Pedraz)

To develop and evaluate a non-viral genome-editing platform, based on auto-assembled lipid nanoparticles and CRISPR/Cas9 technology


Development and optimization of anti-inflammatory nanotherapies in inherited retinal dystrohies

Gustavo Puras Ochoa (Pedraz)

Nanoformulations carrying potential therapeutic agents


Heart-on-chip: mechano-electro maturation of iPSC and modeling of hypertrophy cardiomyopathy

Ángel Raya

A platform based on organ-on-chip technology to promote the differentiaton and maturation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). An advanced culture system as a tool to study the pathogenic mechanisms underlying hypertrophic cardiomyopathy (HCM).


Development of human iPS-derived neural cells as effective drug discovery model for Lafora´s Disease

Ángel Raya

Platform to monitor stage, progression and effect of drugs on LD


Investigating genetic and mechanistic interactions in familial cardiomyopathy through advanced disease modeling

Ángel Raya

to elucidate the pathogenic mechanisms that link hypertrophic cardiomyopathy (HCM) and left ventricular non-compaction (LVNC). Using advanced disease models based on the combination of induced pluripotent stem cell and gene editing technologies, genetic and mechanistic interactors linking both hereditary cardiomyopathies will be identified.


CRISPR screen of early driver events contributing to glioblastoma development in CMMRD syndrome

Ángel Raya

to identify early and key driver genes contributing to glioblastoma development in CMMRD syndrome patients using advanced disease models based on the combination of induced pluripotent stem cell and CRISPR/Cas9 technologies.


Pre-clinical assessment of human iPSC-derived cell therapy in a large animal model of myocardial infarction

Ángel Raya

optimized culture systems for large-scale cardiomyocyte production. The use of swine model of myocardial infarction will offer the best pre-clinical assessment of the functionality and safety of injecting human iPSC-derived cardiomyocytes.


Development of targeted nanotherapy against melanoma and HER2 breats cancer

Carlos Rodríguez Abreu

The nanoparticles developed in this project constitute a therapeutic platform that will be validated by in vitro and in vivo preclinical models


Validating multifunctional polymeric nanoparticles as novel therapeutic vehicles for Alzheimer's Disease

Carlos Rodríguez Abreu, Santiago Grijalvo

The nanoparticles developed in this project constitute a therapeutic platform that will be validated by in vitro and in vivo preclinical models


Mitochondria-Targeting Dendron-Ceria Nanoparticles in the Management of Alzheimer’s Disease

Carlos Rodríguez Abreu

Novel nanopharmaceutics for searching new therapeutic approaches in neurodegenerative diseases like Alzheimer disease with high potential for technology transfer

3D Fibrelaskin

3D Hybrid ELR-Fibrin elastic xenografts for damaged and wounded skin regeneration.

Israel González de Torre (Rodríguez Cabello)

development of a printable 3D system for the creation of artificial skin based on a bioactive, multi-cymponent scaffold.


In vitro cell culture platforms based on injectable hydrogels with affinity to growth factors for hepatotoxicity studies

Mercedes Santos García (Rodríguez Cabello)

Cell culture platform able to predict drug-induced liver injuries based on Interpenetrated Gelatin and ELR Networks containing heparin and growth factors.


Biohybrid Elastin-like/Lactic Acid Hydrogels for Cardiac Tissue Engineering

José Carlos Rodríguez Cabello, Sergio Acosta

new platform for in vitro 3D culture of cardiomyocytes to improve their regenerative potential. To achieve this objective, a multivalent hydrogel (cell adhesive and biodegradable) will be engineered to control the dosage of nutrients and that can be applied to different cell types.

OA-BioMark 2

Development and validation of methods for the evaluation of osteoarthritis-related protein biomarkers useful for its diagnosis, monitoring and therapy

Cristina Ruiz

Protein biomarkers for osteoarthritis: the cartilage intermediate layer protein 1 and the proteoglycan 4


Development of a targeted proteomics method useful to improve the diagnostic accuracy of cardiac amyloidosis and facilitate precision medicine approaches

Cristina Ruiz

Highly specific classification and characterization of amyloidogenic candidates in samples from cardiac or extracardiac biopsies


Deciphering key cell-matrix interactions in differentiation and disease of the musculoskeletal system tissues

Josep Samitier

Biomimetic scaffolds or constructs acting both as models for disease study and as biocompatible/biodegradable implants for musculoskeletal tissue regeneration


Mechanotherapy approaches on 3D in vitro biomimetic neuroblastoma models

Josep Samitier

- In vitro 3D silk-VN composite scaffolds
- Decellularized stroma cell- derived ECM


Blood-brain barrier-on-a-chip (BBB-oC) for drug testing and physiopathological studies

Josep Samitier

Complete model of the BBB with brain tissue and with an integrated electronic system for in situ cellular monitoring/actuation, operating simultaneously with conventional optical evaluation. This platform will be validated in several biological scenarios and it will be developed optimizing the scalability, performance, and cost, while maximizing its ease of use taking into acoumnt the targeted end-users.


Determination of the nanoscale electrical properties of olfactory receptors (ORs) and their dependence on ligand binding: Towards the development of capacitance-operated odorant biosensors

Josep Samitier

New biohybrid electronic detectors based on capacitance-operated odorant biosensors, bearing high sensitivity and selectivity for the detection of volatile organic compounds.


Efficient delivery of inhaled nanoparticles. Development of a pressure pulse nanoaerosol generator for pulmonary drug delivery of antibiotics

Jesús Santamaría

A more easy to handle, and compact device base, suitable for use in the clinic or home care, based on the existing prototype of the innovative nanoaerosol generator patend by University of Zaragoza. This device is able to deliver inhaled pharmaceuticals as a dry powder overcoming most of the drawbacks of commertial PDI inhalers


Real time detection of COVID-19 presence in enclosed environments using SERS

Jesús Santamaría

A biosensor for real-time detection of SARS-Cov-2 in air


Cancer Stroma Assessment through Digital Pathology

María Jesús Ledesma (Santos)

Digital pathology in characterizing the tumor stroma


Image based biomarkers for Immunotherapy response prediction in Lung cancer

María Jesús Ledesma (Santos)

New spatio-temporal methodologies based on DL (deep-learning) architectures


Lung parenchymal lesion characterization for the study of respiratory diseases

María Jesús Ledesma (Santos)

Image processing techniques to harmonize the images in time and assess properly the quantitative assessment of parenchymal lesions evolution


Quantification of SPECT and PET images in Parkinson’s disease and degenerative parkinsonisms

Aida Niñerola (Setoain)

Quantification algorithms, quantification results and SPECT/PET images database


Neurological mechanisms of resilience to Alzheimer´s Disease

Juan Domingo Gispert (Setoain)

Imaging outcomes


Cell proliferation in pulmonary hypertension: Detection and mechanisms of epigenetic regulation

Aida Niñerola (Setoain)

Quantification results


Validation of quantitative susceptibility mapping for cortical iron deposits quantification in Parkinson's disease

Raúl Tudela (Setoain)

Quantification results


PET/CT in patients with lymphoma treated with CAR-T therapy

Francisco Javier Setoain

PET/CT images, analized with radiomic features, may predict treatment response before CART infusion in lymphoma


New software for the generation of realistic brain PET and SPECT images

Aida Niñerola (Setoain)

Software for simulation. Web application


Application of PET and SPECT images in cardiovascular infections

Andrés Perissinotti (Setoain)

PET and SPECT clinical use algorithm and imaging processing techniques for the diagnosis and monitoring of cardiovascular infections such as prosthetic valve endocarditis, TAVI endocarditis, cardiac implantable electronic device infection, vascular graft infection and their complications


Improving image processing and quantification for brain MRI and PET in neurodegenerative disorders.

Roser Sala-Llonch (Setoain)

Software for automated quantification, image processing and analysis. Web application.


Mechanobiology of colorectal cancer tumor organoids

Xavier Trepat

Web application.


Mechanical variables driving mechanosensing

Xavier Trepat

infusion. The knowledge of this network would provide valuable information in the interpretation of FDG PET/CT scans of


Organic radicals and radical dendrimers to improve the magnetic resonance imaging

José Vidal (Ventosa)

these patients, and could help design treatment strategies in the context of relapsed and refractory disease.


3D scaffolds and dynamic molecular bio-interfaces for controlled environments towards vascular morphogenesis 

Imma Ratera (Ventosa)

- Substrates and 3D porous scaffolds functionalized with self-assembled monolayers of electroactive molecules with GF-IBs and RGD and VEGF derived peptides and dendrimers
- Nanostructured cationic polymers interfaces to trap negatively charged DNA


Quatsome: stable nanovesicles for nucleic acids delivery

Nora Ventosa

- Pegilated-molecular units encapsulated in Quatsomes membrane (stealth QTs)
- iRNA: Stealth QTs functionalized with peptides that target the colorectal cancer or neuroblastoma receptors


Tailored Quastomes and Coatings for the Treatment of skin disorders and Infections

Nora Ventosa

novel treatments for a wide variety of skin disorders, including healing
of superficial cutaneous lesions such as scars, chronic inflammatory skin disorders with complex etiology including defective
skin barrier function, allergen sensitization, and recurrent skin infections, such as atopic dermatitis, and the healing of
complex wounds (diabetic foot ulcers, venous ulcers, etc.) of grade I and II. Moreover, the results will also translate into easy
tunable coatings (wound dressings and catheters) with a superior biocompatibility and improved antimicrobial activity.


New Fluorescent Organic Nanoparticles for the multiplex detection of antigens

Nora Ventosa

Fluorecently-labelled Quatsomes (nanovesicles composed of quaternary ammonium surfactant and cholesterol) with antibodies incorporated in their membrane


3D printed artificial immune tissues on a chip for adoptive cell therapy

Judith Guasch (Ventosa)

The fabrication of an “immune tissue-on-a-chip”


Studying the intricacies of active targeting in nanoliposomal formulations

Nora Ventosa, Mariana Köber

In-depth characterization and optimization of the targeting efficacy of different nanoliposomal formulations


NIR-responsive scaffolds for biomedical applications

Nuria Vilaboa

NIR-responsive scaffolds based on fibrin/cryogels/recombinant elastomers and gold or Cu NIR nanotransducers that include thermosensitive liposomes, niosomes containing rapamycin or transgenic cells harboring osteogenic/angiogenic genes


Browning and whitening adipose cells on-chip

Xavier Muñoz Berbel (Villa)

Laboratory tool exploiting the lab-on-a-chip concept for culturing, in-situ stimulation and analysis of adipose cells


Chemical Functionalization Strategies of Graphene Solution Gated Field Effect Transistors with Oligonucleotides for Biosensing

Rosa Villa, Elisabet Prats

to increase the highthrougput and the reliability of gSGFETs to develop a useful tool for the detection of gene sequences related to specific pathologies. The development of new chemical strategies to selective functionalizations in order to allow multi-analyte detection, and the development of chemical methodologies to study the gSGFET behavior in deep and increase the reliability of this sensors, can be translated into successful achievements that increase the knowledge towards the sensing field. This knowledge can boost the development of sensing platforms that enlarge the capabilities of the existing molecular techniques for gene sensing.


Towards a quantitative assessment of bipolar disorder

Jordi Aguiló (Villa)

Procedure or device to objectively capture disease activity in bipolar disorder. To develop a multiparametric model including objectifiable variables mainly obtained from electrophysiological signals recorded from a wearable device


mHealth Methods in Mental Health Research: Designing an Objective Tool to Assess Mental Health in Epidemiologic and Clinical Studies

Jordi Aguiló (Villa)

Procedure or device to assess the degree of mental well-being.


Avoiding and reducing stress to improve transplant surgery and recovery

Jordi Aguiló (Villa)

Adaptation and validation of a tool for pre-surgical stress prevention in transplant patients. SAM app will be adapted, validated and evaluated for transplant


Design of tumor-targeted nanostructured venoms for the treatment of CXCR4+ human neoplasias

Antonio Villaverde

Protein-only CXCR4-targeted nanoparticles


Prelinical development of a novel protein-based nanobiotechnological platform for the remote delivery of proteins with antitumor activity

Esther Vázquez (Villaverde)

Inclusion Bodies formed by fluorescent or cytotoxic proteins that target CXCR4


Targeted Reinforcement of the Innate immune response Against Leukemic Stem cells

Eloi Parladé Molist (Villaverde)

Antitumoral site-directed drugs based on protein nanoparticles and aimed to boost innate immune response against metastatic cancer stem cells in CXCR4+ human neaoplasias, especially AML


Enzyme mutants as tools in the quest for improved therapies for Fabry disease

Neus Ferrer (Villaverde)

Improved nanoformulation of GLA enzyme for a better Fabry disease therapy. This will rely in two main aspects: i) engineering of GLA to obtain mutants with a higher enzymatic activity than wild-type enzyme, and ii) the nanoformulation of such improved mutants by their assembly into nanoparticles with a better pharmacokinetics than free, soluble GLA and that could act as protein depots for a controlled, continuos release of the needed GLA enzyme in Fabry patients.


 Multifunctional protein-only nanoparticles for nose-to-brain delivery

Esther Vázquez (Villaverde)

 Multifunctional protein-only nanoparticles for nose-to-brain delivery


Structure and antigenicity of SARS-COV-2 antigens formulated as self-assembling nano- and micro-particles

Eloi Parladé Molist (Villaverde)

A new formulation of SARS-CoV-2 antigens as slow antigen delivery system for immune protection. Transversal technology of applicability to other infectious diseases